Detail Article

Material Dental Journal / Vol. 8. No. 2. 2018-04: 41 - 46


Antibacterial potency of mixed Piper crocatum extract and zinc oxide against of Enterococcus faecalis


Daya antibakteri campuran ekstrak Piper crocatum dan zinc oxide terhadap bakteri Enterococcus faecalis

  1. Sesy Ayu Lestari
    Mahasiswa
    sesyayu@gmail.com
  2. R. Helal Soekartono
    Staff Dosen FKG Unair
    mashelal@gmail.com
  3. R. Mohammad Yogiartono
    Staff Dosen FKG Unair
    drg_yogiartono@yahoo.co.id

Abstract

Enterococcus faecalis is a anaerob facultative gram-positive bacterial often found in infection after root canal treatment. Zinc oxide eugenol is one of the root canal filling which is still often used. Over filling of eugenol can cause irritation. Piper crocatum has more antibacterial properties that contained some of the active compounds. There were flavonoids, alkaloids, tannins, saponin, polifenolat, fenol and its derivatives. The extract of Piper crocatum expected to be used as an alternative material eugenol. Purpose: The aim of this study was to determine antibacterial activity of a mixture of Piper crocatum extract and zinc oxide in killing E. faecalis bacteria at the concentrations of 50%, 37.5%, 25% and 12.5%. Methods: The extraction of Piper crocatum was made by macerating with 96% of ethanol and divided into four concentrations. Zinc oxide powder is mixed with an extract of Piper crocatum a ratio of 1:1, then a suspension of bacteria E. faecalis which was comparable to a McFarland standard 0.5 of 1.5 x 108 cfu/µ was made. Six petridish containing Blood Agar media were divided into 4 sections each petridish. The mixture of Piper crocatum extract with some concentrations of zinc oxide bubuk was applied to the Blood Agar media with diffusion method. The results were analyzed with one-way Anova test and Tukey HSD test. Results: Significant differences were found between the inhibition zone on all treatment groups in inhibiting bacteria E. faecalis. Conclusions: The mixture of Piper crocatum extract and zinc oxide has antibacterial activity in killing E. faecalis bacteria with the minimum concentration is 12.5% and the highest at concentration of 50%.

Keyword: Piper crocatum, zinc oxide, E. faecalis,

Abstrak

Enterococcus faecalis adalah bakteri kokus gram positif fakultatif anaerob yang sering ditemukan pada infeksi pasca perawatan saluran akar. Zinc oxide eugenol merupakan salah satu bahan pengisi saluran akar yang masih sering digunakan. Pengisian eugenol yang berlebih dapat menyebabkan iritasi. Piper crocatum memiliki sifat antibakteri yang terdapat dalam beberapa senyawa aktif yaitu flavonoid, alkaloid, tanin, saponin, polifenolat, fenol dan derivatnya. Ekstrak Piper crocatum diharapkan dapat digunakan sebagai bahan alternatif eugenol. Tujuan: Untuk mengetahui daya antibakteri campuran ekstrak Piper crocatum dan zinc oxide dalam membunuh bakteri Enterococcus faecalis pada konsentrasi 50%, 37,5%, 25% dan 12,5%. Metode: Pembuatan ekstrak Piper crocatum dilakukan dengan cara maserasi menggunakan etanol 96% dan dibuat 4 konsentrasi. Bubuk zinc oxide dicampur dengan ekstrak Piper crocatum dengan perbandingan 1:1. Suspensi bakteri E. faecalis disetarakan dengan standar McFarland 0,5 sebesar    1,5 x 108 cfu/µ. Enam petridish berisi media blood agar dibagi menjadi 4 bagian setiap petridish. Campuran ekstrak Piper crocatum beberapa konsentrasi dengan bubuk zinc oxide diaplikasikan ke media blood agar dengan metode difusi. Data penelitian dianalisis menggunakan one way Anova test serta Tukey HSD test.  Hasil: Terdapat perbedaan yang signifikan antara zona hambat pada seluruh kelompok perlakuan dalam menghambat bakteri E. faecalis. Simpulan: Campuran ekstrak Piper crocatum dan zinc oxide memiliki daya antibakteri dalam membunuh bakteri E. faecalis dengan konsentrasi minimal 12,5% dan yang paling tinggi pada konsentrasi 50%.


Daftar Pustaka

  1. Peciuliene V, Isolation of yeast and enteric bacteria in root-filled teeth with chronic apical periodontitis. -, Int Endod J. 2001; 34: 429-34, 2001.
  2. Reynaus AH, Isolation of yeast and enteric bacteria in root-filled teeth with chronic apical periodontitis . -, Int Endod J. 2001; 34: 429-34, 2001.
  3. Balciuniene I, Isolation of yeast and enteric bacteria in root-filled teeth with chronic apical periodontiti. -, Int Endod J. 2001; 34: 429-34, 2001.
  4. Suchitra U, Enterococcus faecalis: an endodontic pathogen. -, MedInd Journals. 2006; 18(2): 12, 2006.
  5. Kundabala M, Enterococcus faecalis: an endodontic pathogen. -, MedInd Journals. 2006; 18(2): 12, 2006.
  6. Ameliana Y, Daya antibakteri penambahan propolis pada zinc oxide eugenol dan zinc oxide terhadap kuman campur gigi molar sulung non vital. Surabay, Dental Journal Majalah Kedokteran Gigi. 2014; 47(4): 198-201, 2014.
  7. Chawla HS, Evaluation of a mixture of zinc oxide, calcium hydroxide, and sodium fluoride as a new root canal filling material for primary teeth. India, The Indian Social Pedodontic Preventive Dentistry Journal. 2008; 26(2): 53, 2008.
  8. Setia S, valuation of a mixture of zinc oxide, calcium hydroxide, and sodium fluoride as a new root canal filling material for primary teeth. India, The Indian Social Pedodontic Preventive Dentistry Journal. 2008; 26(2): 53, 2008.
  9. Gupta N, Evaluation of a mixture of zinc oxide, calcium hydroxide, and sodium fluoride as a new root canal filling material for primary teeth. India, The Indian Social Pedodontic Preventive Dentistry Journal. 2008; 26(2): 53, 2008.
  10. Gauba K, Evaluation of a mixture of zinc oxide, calcium hydroxide, and sodium fluoride as a new root canal filling material for primary teeth. India, The Indian Social Pedodontic Preventive Dentistry Journal. 2008; 26(2): 53, 2008.
  11. Arora DS, Bhardwaj. Antibacterial activity of some medicinal plants. -, Geo Bios: 1997. p. 127-31, 1997.

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