Detail Article

Oral Medicine Dental Journal / Vol. 9. No. 1. 2017-01: 66 - 71


Citotoxicity Effects of Citrus limon Peel Essential Oil in BHK-21 Fibroblast


DAYA SITOTOKSISITAS MINYAK ESENSIAL KULIT BUAH CITRUS LIMON TERHADAP SEL FIBROBLAS BHK-21

  1. Sela Yuni Putriana
    Mahasiswa S1 Pendidikan Dokter Gigi
    sela.yuni-13@fkg.unair.ac.id
  2. Priyo Hadi
    Staff Pengajar Departemen Ilmu Penyakit Mulut
    Priyohadi11@yahoo.com
  3. Desiana Radithia
    Staff Pengajar Departemen Ilmu Penyakit Mulut
    deisy.radithia@fkg.unair.ac.id

Abstract

Background: The Citrus limon peel essential oil is one of herbal medicine that have been reported as antiinflammatory, antibacterial, antifungal, antioxidant and anticancer. The chemical compounds such D-Limonene, α-Pinene, β-Pinene dan γ-Terpinene has been proven to have antioxidant activity. However, the research of its toxicity as therapeutic substance has not been done. It is the underlying need to testing the citotoxicity of materials in dentistry. Objective: The aim of this study to determine the toxicity effect of essential oil Citrus limon to BHK-21 fibroblast. Methods: Experimental laboratory in vitro study of BHK-21 fibroblast. The Citrus limon peel essential oil was made by steam distilation and diluted using serial dilution with addition of Tween 20 0.05% + corn oil. The cytotoxicity was conducted by MTT assay and the optical density was measured using ELISA Reader at 620 nm wavelength. Then, the optical density values ​​were calculated using the formula for determining the number of survival fibroblasts after tested. Results: Data was analyzed using Post Hoc Tukey HSD that showed there is no significancy at concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.56%. At concentration 0.78% it showed significant different (p > 0.05). Conclusion: The Citrus limon peel essential oil has cytotoxic effects at concentration of 100% to 1.56%.

Keyword: Citrus limon peel essential oil, Citotoxicity, BHK-21 fibroblast,

Abstrak

Latar Belakang: Minyak esensial kulit buah Citrus limon merupakan salah satu bahan herbal yang telah banyak dilaporkan memiliki sifat antiinflamasi, antibakteri, antifungi, antioksidan dan antikanker. Kandungan senyawa kimia seperti D-Limonene, α-Pinene, β-Pinene dan γ-Terpinene diketahui memiliki sifat antioksidan. Untuk mengembangkan minyak esensial kulit buah Citrus limon sebagai bahan terapetik pada bidang kedokteran gigi, diperlukan suatu uji bahan, salah satunya uji sitotoksisitas. Tujuan: Penelitian ini bertujuan untuk mengetahui efek sitotoksisitas minyak esensial kulit buah Citrus limon terhadap sel fibroblas BHK-21. Metode: Penelitian eksperimental laboratoris dilakukan secara in vitro dengan menggunakan kultur sel fibroblas BHK-21. Minyak esensial Citrus limon didapat melalui metode distilasi uap dan diencerkan secara berseri menggunakan Tween 20 0.05% + corn oil. Uji sitotoksisitas dilakukan dengan metode MTT assay dan penghitungan optical density menggunakan ELISA Reader pada panjang gelombang 620 nm. Nilai optical density yang didapat kemudian dihitung menggunakan rumus untuk menentukan viabilitas sel fibroblas setelah perlakuan. Hasil: Analisa data menggunakan Post Hoc Tukey HSD menunjukkan tidak ada perbedaan signifikan pada kelompok konsentrasi 50%, 25%, 12.5%, 6.25%, 3.125%, dan 1.56%. Perbedaan yang bermakna didapatkan pada konsentrasi 0.78% (p > 0.05). Simpulan: Minyak esensial kulit buah Citrus limon memiliki efek sitotoksik pada konsentrasi 100%-1.56%.


Daftar Pustaka

  1. Mohamed, A.A, Influence of Some Citrus Essential Oils on Cell Viability, Glutathione-S-Transferase and Lipid Peroxidation in Ehrlich ascites Carcinoma Cells. -, Journal of American Science, 2010.
  2. El-Emary, Influence of Some Citrus Essential Oils on Cell Viability, Glutathione-S-Transferase and Lipid Peroxidation in Ehrlich ascites Carcinoma Cells. -, Journal of American Science, 2010.
  3. Ali, H.F, Influence of Some Citrus Essential Oils on Cell Viability, Glutathione-S-Transferase and Lipid Peroxidation in Ehrlich ascites Carcinoma Cells. -, Journal of American Science, 2010.

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