Detail Article

Oral Medicine Dental Journal / Vol. 9. No. 1. 2017-01: 72 - 76


Citrus limon Peel Essential Oil Inhibitory Effect Against Candida Albicans Biofilm Growth


DAYA HAMBAT MINYAK ESENSIAL KULIT BUAH CITRUS LIMON TERHADAP PERTUMBUHAN BIOFILM CANDIDA ALBICANS

  1. Wiwin Saputri
    Mahasiswa S1 Pendidikan Dokter Gigi
    wiwin.saputri-13@fkg.unair.ac.id
  2. Desiana Radithia
    Staff Pengajar Departemen Ilmu Penyakit Mulut
    deisy.radithia@fkg.unair.ac.id
  3. Priyo Hadi
    Staff Pengajar Departemen Ilmu Penyakit Mulut
    Priyohadi11@yahoo.com

Abstract

Background: Candida albicans is a common flora on human body mucosa that can turn into an opportunistic pathogen and cause candidiasis, mostly in immunocompromised patients. Candida albicans produces biofilm to protect themselves from antifungal therapy, causing resistance against standard therapy. Citrus limon peel contains active ingredients which should be able to inhibit Candida albicans biofilm growth. Objective: To observe the inhibitory effects of Citrus limon peel essential oil against Candida albicans biofilm growth. Method: Candida albicans biofilms grown in SDB with  8%glucose concentration, then treated with Citrus limon peel essential oil of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56% and 0.78% concentration. Staining with crystal violet 0.1% was performed for 15 minutes and followed by reading the optical density using a microplate reader at 595 nm wavelength. Results: The lowest inhibitory effects of Citrus limon peel essential oil is at 3.125% concentration. At 0.78% to 25% concentration, there were no significant difference. At 50% and 100% concentration, there was significant difference. Conclusion: At 0,78% concentration the Citrus limon peel essential oil had been able to inhibit Candida albicans biofilm growth and the significant effect was observed at 50% to 100% concentration.

Keyword: Candida albicans , Biofilm, Citrus limon peel essential oil,

Abstrak

Latar Belakang: Candida albicans adalah flora normal pada permukaan mukosa manusia yang dapat bersifat patogen oportunistik dan menyebabkan Candidiasis, terutama pada pasien imunokompromis. Biofilm Candida albicans digunakan untuk melindungi diri dari terapi antifungi, sehingga timbul resistensi terhadap terapi standar. Kulit buah Citrus limon diperkirakan memiliki kandungan bahan aktif yang mampu menghambat pertumbuhan biofilm Candida albicans. Tujuan: Untuk mengamati daya hambat minyak esensial kulit buah Citrus limon terhadap pertumbuhan biofilm Candida albicans. Metode: Biofilm Candida albicans ditumbuhkan pada SDB dengan konsentrasi glukosa 8%. Lalu diberi perlakuan dengan minyak esensial kulit buah Citrus limon konsentrasi 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56% dan 0,78%. Selanjutnya, dilakukan pewarnaan menggunakan crystal violet 0,1% selama 15 menit dan dilanjutkan dengan pembacaan optical density  menggunakan microplate reader pada panjang gelombang 595 nm. Hasil: Daya hambat minyak esensial kulit buah Citrus limon terendah ada pada konsentrasi 3,125%. Pada konsentrasi 0,78% sampai 25% tidak menunjukkan perbedaan yang signifikan. Pada konsentrasi 50% dan 100% mulai nampak perbedaan signifikan. Simpulan: Pada konsentrasi 0,78%  minyak esensial kulit buah Citrus limon sudah mampu menghambat pertumbuhan biofilm Candida albicans, dan daya hambat signifikan ada pada rentang konsentrasi 50% sampai 100%.


Daftar Pustaka

  1. Leite, MCA, Evaluation of Antifungal Activity and Mechanism of Action of Citral against Candida albicans’. -, Evidence-Based Complementary and alternative medicine, 2014.
  2. Bezerra, APD, Evaluation of Antifungal Activity and Mechanism of Action of Citral against Candida albicans’. -, Evidence-Based Complementary and alternative medicine, 2014.
  3. Sousa, JP, Evaluation of Antifungal Activity and Mechanism of Action of Citral against Candida albicans’. -, Evidence-Based Complementary and alternative medicine, 2014.

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