Detail Article

Oral Biology Dental Journal / Vol. 5. No. 1. 2013-01: -


Cytotoxicity Test of Ethanolic Extract of Red Betel Leaf (Piper crocatum) Toward Fibroblast Cells


Uji Sitotoksisitas Ekstrak Etanol Daun Sirih Merah (Piper crocatum) terhadap Sel Fibroblas

  1. Pelangi Citra Pramita Sari
    Pendidikan Dokter Gigi
    -
  2. Markus Budi Rahardjo
    Staf Pengajar Departemen Biologi Oral Fakultas Kedokteran Gigi Universitas Airlangga
    -
  3. Ira Arundina
    Staf Pengajar Departemen Biologi Oral Fakultas Kedokteran Gigi Universitas Airlangga
    -

Abstract

Background. Traditional herbs are very popular in the community lately as a natural substance that is considered more safe, inexpensive anda easy to obtain. Piper crocatum is one of the Indonesian plants which is traditionally used for health treatment. It is known to have anti-inflammatory and wound healing effect. The using Piper crocatum as one of the dental health products should have not toxic, and have biocompatibilty properties. Purpose. To determine the cytotoxicity of the Piper crocatum with different concentration toward fibroblast cells.. Method. The method type used is experimental research laboratories using the post-test only control group design. Number of samples were 7 samples of each 12,5%, 25%, 50%, and 100%. The citotoxicity test by using enzymatic assay of MTT [3-(4,5-dimethyltiazol-2-yl)-2,5-difeniltetrazolium bromide], against fibroblast cell (BHK-21). The density of optic formazan indicated the number of living cell. Results. The result showed that the percentage of living cell amount of 6,25%, 12,5%, 25%, 50% and 100% concentration were 63,47%, 52,52%, 56,21% and 100%. Conclusion. Piper crocatum is non toxic toward fibroblast cells.

Keyword: Piper crocatum, cytotoxicity, MTT assay,

Abstrak

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Daftar Pustaka

  1. Vegad JL, A Textbook of Veterinary General Pathology; Healing and Repair. New Delhi, A Textbook of Veterinary General Pathology; Healing and Repair, 1995.

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