Detail Article

Journal Media Material Dental Journal / Vol. 3. No. 2. 2012-07

Table of Content : 3 | Page :41 - 46


Effectiveness of brotowali leaf extract as a denture cleanser towards the amount of colonies of Candida albicans


Efektivitas ekstrak daun brotowali sebagai pembersih gigi tiruan terhadap jumlah koloni Candida albicans

  1. Yennata Saputra
    Mahasiswa Program Sarjana Kedokteran Gigi
    yennata saputra@hotmail.com
  2. Asti Meizarini
    Staf Departemen Material Kedokteran Gigi Fkg Unair
    -
  3. Devi Rianti
    Staf Departemen Material Kedokteran Gigi Fkg Unair
    -

Abstract

Background: Individuals who wear denture often experience denture stomatitis, which is an infection of mucosal tissue in contact with denture in the oral cavity. Denture stomatitis associated with Candida albicans proliferation contained on the plaque attached to the denture. Prevention of denture stomatitis can be done by cleaning the denture using denture cleanser. Indonesia is rich in medicinal plants that can be used as an alternative denture cleanser. One of these plants is brotowali (Tinospora crispa Miers). Purpose: The purpose of this study was to determine the effectiveness of brotowali extract as a denture cleanser on the number of colonies of Candida albicans. Method: The study was carried out on 48 specimens of unpolished acrylic resin plates with 10x10x1mm dimension, solution with 20%, 22,5%, 25%, 27,5%, 30%, 32,5%, and 35% brotowali leaf extract concentrations, 15 minutes of immersion times to calculate the Candida albicans colonies. Sterilized aquadest used as a control. Each group consist of 6 specimens. Calculation on Candida albicans existence was carried out by calculating the amount of Candida albicans colonies which grew on Saboraud’s dextrose agar. The data was analyzed using One-Way ANOVA and LSD with 5% degree of significance. Result: The amount of Candida albicans colonies were significantly decreased with increasing concentration of brotowali leaf extract. Conclusion: Concentration of brotowali leaf extract (Tinospora crispa Miers) as a denture cleanser that effectively reduce the number of colonies of Candida albicans present in concentration of 32,5%.

Keyword: Denture stomatitis, resin akrilik,

Abstrak

Latar belakang: Pemakai gigi tiruan sering mengalami denture stomatitis. Denture stomatitis dihubungkan dengan proliferasi Candida albicans dalam plak yang melekat pada gigi tiruan. Pencegahan denture stomatitis dapat dilakukan dengan membersihkan gigi tiruan menggunakan denture cleanser. Indonesia kaya akan tanaman obat yang dapat digunakan sebagai alternatif denture cleanser. Salah satu tanaman tersebut adalah brotowali (Tinospora crispa Miers). Tujuan: untuk mengetahui efektivitas ekstrak brotowali sebagai denture cleanser dengan melihat pertumbuhan jumlah koloni Candida albicans. Metode: Delapan kelompok penelitian, terdiri dari 6 spesimen lempeng resin akrilik yang tidak dipulas, ukuran 10x10x1mm, berjumlah 48 spesimen. Spesimen direndam 15 menit dalam ekstrak daun brotowali konsentrasi 20%, 22,5%, 25%, 27,5%, 30%, 32,5%, dan 35%. Akuades steril digunakan sebagai kontrol. Koloni Candida albicans yang tumbuh pada Saboraud’s dextrose agar pada setiap konsentrasi dihitung, Data di analisis menggunakan ANOVA satu arah dan LSD dengan derajat kemaknaan 5%. Hasil: Jumlah koloni Candida albicans menurun secara bermakna dengan meningkatnya konsentrasi ekstrak daun brotowali. Simpulan: Konsentrasi ekstrak daun brotowali 32,5%.sebagai denture cleanser efektif menurunkan koloni Candida albicans.


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