Detail Article

Material Dental Journal / Vol. 8. No. 1. 2017-01: 17 - 22


Identification of functional groups of chitosan-gelatin and hydroxyapatite composite using FTIR spectroscopy


Identifikasi gugus fungsi komposit kitosan-gelatin dan hidroksiapatit dengan spektroskopi FTIR

  1. Dita Saraswati Suwardi
    Mahasiswa Program Sarjana Kedokteran Gigi
    anita-y@fkg.unair.ac.id
  2. Anita Yuliati
    Staf Departemen Material Kedokteran Gigi
    anitarusalan@hotmail.com
  3. Mohammad Yogiartono
    Staf Departemen Material Kedokteran Gigi
    drg_yogiartono@yahoo.co.id

Abstract

Background: Chitosan, gelatin, and hydroxyapatite as a scaffold material has the advantage of antibacterial, low toxicity, biodegradable, biocompatible with human tissue, facilitating the process of regeneration on wound healing, osteoconductive, and stimulates cell formation in vitro and in vivo. Fourier Transform Infrared Spectroscopy (FTIR) is one of the good analysis techniques in the identification of functional groups of a compound structure. Purpose: Knowing the functional groups of chitosan, gelatin, hydroxyapatite and composite chitosan-gelatin hydroxyapatite (K-G: HA) ratio of 30:70 (w / w) by FTIR. Method: Composite synthesized from chitosan, gelatin, and hydroxyapatite with 3 different ratio K-G:HA 30:70   (w / w) as a control, 20:80 (w / w) and 40:60 (w / w) were tested by FTIR. Results: FTIR test results showed that FTIR spectra obtained on every variation of functional groups OH (hydroxyl group), amide I, amide II, and PO43- ions. Conclusions: Composite chitosan, gelatin and hydroxyapatite containing amide groups (C-N) as a marker ties between chitosan, gelatin and hydroxyapatite

Keyword: Chitosan-gelatin, hydroxyapatite, scaffold, FTIR,

Abstrak

Latar belakang: Kitosan, gelatin, dan hidroksiapatit sebagai material scaffold mempunyai kelebihan antibakteri, toksisitas rendah, biodegradabel, biokompatibel dengan jaringan manusia, mempunyai kemampuan untuk memfasilitasi proses regenerasi terhadap penyembuhan luka, mempunyai kemampuan osteokondutif, dan merangsang pembentukan sel secara in vitro maupun in vivo. Spektroskopi Fourier Transform Infrared (FTIR) merupakan salah satu teknik analisis yang baik dalam proses identifikasi struktur gugus fungsi suatu senyawa. Tujuan: Mengetahui gugus fungsi kitosan, gelatin, hidroksiapatit dan komposit kitosan-gelantin dan hidroksiapatit (K-G:HA) perbandingan 30:70 (w/w) menggunakan spektroksopi FTIR.  Metode: Komposit dari kitosan-gelatin, dan hidroksiapatit (K-G:HA) disintesis dengan rasio yang berbeda yaitu 30:70 (w/w) sebagai kontrol, 20:80 (w/w), dan 40:60 (w/w) kemudian diuji dengan FTIR. Hasil: Hasil menunjukkan bahwa spektrum FTIR pada setiap variasi perbandingan didapatkan gugus fungsi –OH (gugus hidroksil), amida I, amida II, dan ion PO43-. Simpulan: Komposit kitosan-gelatin dan hidroksiapatit mengandung gugus amida (C-N) sebagai marker adanya ikatan antara kitosan, gelatin dan hidroksiapatit


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