Detail Article

Material Dental Journal / Vol. 8. No. 1. 0000-00: 11 - 16


Minimum bactericidal concentration of papaya seed (Carica papaya Linn. var. Bangkok) extract against Streptococcus viridans


Konsentrasi bunuh minimal ekstrak biji pepaya (Carica papaya Linn. var. Bangkok) terhadap Streptococcus viridans

  1. Efata Dewi Nurmalitasari
    Mahasiswa Program Sarjana Kedokteran Gigi
    lita.efata@gmail.com
  2. Priyawan Rachmadi
    Staf Pengajar Departemen Material Kedokteran Gigi
    rachmadi.pri@gmail.com
  3. Intan Nirwana
    Staf Pengajar Departemen Material Kedokteran Gigi
    intannirwana@ymail.com

Abstract

Background: Streptococcus viridans (S. viridans) is the most common bacteria found in infected root canal. Irrigation is a key part of successful root canal treatment. Sodium hypochlorite (NaOCl) is the most common irrigation solution used during root canal therapy. Sodium hypochlorite is a strong base. Material with strong base has higher toxicity to surrounding tissues and lower antibacterial activity. The another weakness of NaOCl include its inability to remove smear layer because its high surface tension. Papaya seed contains alkaloid, flavonoid, tannin, saponin, and papain enzyme. That active substances have been known for their antibacterial activity. Purpose: The aim of this study was to determine the minimum bactericidal concentration (MBC) of papaya seed extract against S. viridans. Methods: Papaya seed extract was made by maceration method with ethanol 96% and dilute into several consentrations (100%, 50%, 25%, 12.5%, 6.12%, 3.12%, 1.56%, and 0.78%). The value of MBC was measured by counting manually the colonies of bacteria on nutrient agar media and applied in Colony Forming Unit/ml (CFU/ml). Data was analyzed statistically by one way ANOVA test and Tukey HSD test. Result:  Based on the colony counting showed that at papaya seed extract 1.56%, there was a decrease in the number of S. viridans bacterial colonies. There was no growth of bacterial colonies at papaya seed extract 3.12%. one way ANOVA test showed there was significant different of the growth of S. viridans at sample groups due to papaya seed extract. Conclusion: Minimum bactericidal concentration value of papaya extract against S. viridans is 3.12%.

Keyword: Minimal, bactericidal, concentration,

Abstrak

Latar belakang: Streptococcus viridians (S. viridans) adalah bakteri yang paling banyak ditemukan pada infeksi saluran akar. Tindakan irigasi merupakan salah satu kunci sukses perawatan saluran akar. Bahan irigasi saluran akar yang banyak digunakan adalah sodium hipoklorit (NaOCl). Sodium hipoklorit mempunyai pH basa bersifat toksik pada jaringan sekitar dan sifat antibakteri rendah. Sodium hipoklorit mempunyai tegangan permukaan tinggi sehingga tidak dapat membersihkan smear layer dengan baik. Biji pepaya mempunyai kandungan alkaloid, flavonoid, tanin, saponin, dan enzim papain, yang berfungsi sebagai bahan antibakteri sehingga diharapkan dapat membunuh bakteri S. viridans. Tujuan: Mengetahui konsentrasi bunuh minimal (KBM) ekstrak biji pepaya terhadap bakteri S. viridans. Metode: Biji pepaya diekstraksi dengan etanol 96% dengan metode maserasi dan diencerkan menjadi beberapa konsentrasi (100%, 50%, 25%, 12,5%, 6,12%, 3,12%, 1,56%, dan 0,78%). Nilai KBM diketahui dengan menghitung jumlah koloni bakteri pada media nutrient agar secara manual dengan satuan Colony Forming Unit/ml (CFU/ml). Data dianalisis dengan uji one way ANOVA dan uji Tukey HSD. Hasil: Pada ekstrak biji pepaya 1,56% mulai ditemukan hambatan pertumbuhan bakteri S. viridans, sedangkan pada ekstrak biji papaya 3,12% sudah tidak ditemukan pertumbuhan bakteri S. viridans. Hasil uji one way ANOVA didapatkan perbedaan yang signifikan pertumbuhan bakteri S. viridans pada kelompok sampel setelah pembrian ekstrak biji pepaya. Simpulan: Konsentrasi bunuh minimal ekstrak biji papaya terhadap bakteri S. viridans sebesar 3,12%.


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