Detail Article

/ Vol. 6. No. 1. 2016-01: 5 - 10

Minimum inhibitation concentration (MIC) and minimum bactericidal concentration (MBC) of henna leafs extract (Lawsonia inermis Linn) against Enterococcus faecalis

Konsentrasi hambat minimal (KHM) dan konsentrasi bunuh minimal (KBM) ekstrak daun pacar kuku (Lawsonia inermis Linn) terhadap Enterococcus faecalis

  1. Cyntia Nur Malikfa Nugraha
    Student of Dentistry, Faculty of Dentistry, Airlangga University, Surabaya
  2. M.Mudjiono
    Departement of Conservative, Faculty of Dentistry, Airlangga University, Surabaya
  3. Febriastuti Cahyani
    Departement of Conservative, Faculty of Dentistry, Airlangga University, Surabaya


Background. The primary causes of root canal treatment failure is a settled bacterial infection which remaining in the root canal and periradikular tissue after the preparation or obturation. Enterococcus faecalis is a bacterium that is found about 77% of cases are resistant to treatment that can causing secondary endodontic infections. At this time, the increase of antibiotic resistance and side effects of synthetic drugs increase research efforts to find alternative herbal ingredients that have antibacterial properties effective and not toxic that can be used as a root canal irrigation. One of herbal antibacterial ingredients that are currently developed are leaves of henna. Antibacterial activity of henna leaf extract against Enterococcus faecalis bacteria can be determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Purpose. To determine MIC and MBC of henna leafs extract against the bacteria Enterococcus faecalis. Method. This research is a laboratory experimental with post test only control group design which used Enterococcus faecalis ATCC 29212 with treatment henna leafs extract on concentration 50%, 45%, 40%, 35%, 30%, dan 25% using Brain Heart Infusion Broth (BHIB). Value of MIC and MBC henna leafs extract were done by calculating manually the growth of bacteria colonies in Nutrient agar  with CFU/ml results. Result. At  the concentration 30% of henna leafs extract, showed that colonies growth less than 10%. At the concentration 35% was not revealed any bacterial growth. Conclusion. The henna leafs extract has antibacterial effect on bacteria Enterococcus faecalis. The MIC was at 30% and MBC was at 35%. 

Keyword: Henna leafs extract, Enterococcus faecalis, Minimum Inhibitory Concretation,



Daftar Pustaka

  1. Shahani, Comparison of Antimicrobial Substantivy of Root Canal Irrigants In Indtrument Root Canal up to 72h : An in vitro study. -, Journal of the Indian Society of Pedodontics & Preventive Denstistry, 2011.
  2. Marcos SE, Quantitive and Qualitative analysis of microorganisms in root-filled teeth with persistent infection : Monitoring of the endodontic retreatment. -, Journal of Restorative Dentistry, Endodontic Division, 2013.
  3. Patidar, Phenotypic Detection of Virulence Traits and Antibiotic Susceptibility of Endodontic Enterococcus faecalis Isolates. -, American Journal of Microbiological Research, 2013.

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