Detail Article

Conservative Dentistry Journal / Vol. 6. No. 1. 2016-01: 21 - 27


Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of Turmeric Extract (Curcuma longa Linn.) against Enterococcus faecalis


Konsentrasi Hambat Minimal dan Konsentrasi Bunuh Minimal Ekstrak Kunyit (Curcuma longa Linn.) terhadap Enterococcus faecalis

  1. Aghnia Alma L
    Mahasiswa Pendidikan Dokter Gigi Fakultas Kedokteran Gigi Universitas Airlangga Surabaya-Indonesia
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  2. Latief Mooduto
    Staf Pengajar Departemen Konservasi Gigi Kedokteran Gigi Fakultas Kedokteran Gigi Universitas Airlangga Surabaya-Indonesia
    latiefmdt@yahoo.com
  3. Devi Eka Juniarti
    Staf Pengajar Departemen Konservasi Gigi Kedokteran Gigi Fakultas Kedokteran Gigi Universitas Airlangga Surabaya-Indonesia
    juniartidevieka@yahoo.com

Abstract

Background.Enterococcus faecalis is one of bacteria that are associated with root canal treatment failure. Elimination of pathogenic microorganisms through root canal irrigation plays important role in root canal treatment.Herbal ingredients have been proven to have antibacterial activity that makes them effective for root canal irrigation, one of them is turmeric (Curcuma longa Linn.). Antibacterial activity of turmeric extract can be observed through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Purpose.The aim of this study was to determine the MIC and MBC of turmeric extract against E. faecalis.Methods.This research was a laboratory experimental study. Turmeric extract was made by maceration method and certain dilution was performed to obtain various concentrations. Values of MIC and MBC of turmeric extract against E. faecalis were known by calculating the growth of bacteria colonies on blood agar media using colony forming unit (CFU).Results.The growth of bacteria colonies at 50% and 55% concentration in sequence are 14,8% and 4,5% of the positive control, whereas at 60% until 100% concentration there was no growth of bacteria colonies. Conclusion.MIC and MBC of turmeric extractagainst E. faecalis are each at 55% and 60% concentration.

Keyword: Turmeric extract , Enterococcus faecalis , MIC,

Abstrak

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Daftar Pustaka

  1. Lakshmi T, Antibacterial activity of two herbal extracts and 2% sodium hypochlorite against Enterococcus faecalis: An invitro comparative study. -, J Chem Pharm Res, 2013.
  2. Mallick R, Enterococcus faecalis: A resistant microbe in endodontics. -, J Contemp Dent Med Rev, 2014.
  3. Gaikwad A, Antibacterial activity of isoflavone extracted from Curcuma longa linn. Zingeberaceae. ISOI . -, Journal of Microbiology, Biotechnology and Food Science, 2014.

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