Detail Article

Oral Biology Dental Journal / Vol. 2. No. 1. 2010-07: -


The content testing of IgG on white rat serum that is applied with glukanase enzyms as a inhibition candidate to the formation of dental plaque)(Inhibitory effect of basil leaf extract (Ocimum sanctum) to the growth supragingiva plaque’s bacteria


Uji Kadar IgG Serum Tikus Putih Yang Diaplikasi Dengan Enzim Glukanase Sebagai Kandidat Penghambat Pembentukan Plak Gigi

  1. Malika,
    Mahasiswa S1 Pendidikan Dokter Gigi
    my_melody_16@yahoo.com
  2. Indah Listiana K,
    Staf Pengajar Departemen Biologi Oral
    Fakultas Kedokteran Gigi Universitas Airlangga
    Surabaya
    -
  3. Afaf Baktir
    Staf Pengajar Departemen Biologi Oral
    Fakultas Kedokteran Gigi Universitas Airlangga
    Surabaya
    -
  4. -
    -
    -
  5. -
    -
    -

Abstract

Background: Glucanase enzymes isolate of bacteria B4, B5 and B7 isolated from decayed wood, soil and mud in vitro have proven effective in preventing dental plaque. Glucanase enzymes are a protein that have the potential to become antigen. The biocompability of these enzymes were tested immunologicaly viewed from immunohumoral response through increasing of IgG level in rat blood serum. Purpose: To identify body humoral immune response on glucanase enzymes exposure from isolate B4, B5, and B7 as candidate of dental plaque formation. Method: To measure rat serum IgG level with Indirect ELISA method viewed from optical density (OD) value by using ELISA Reader in wavelength λ450 m. Results. There were no significant difference on IgG level increase between B4 and B5 groups where (P =0 .171 > a = 0.05). Conclusion: Glucanase enzymes from bacteria isolates B4 and B5 as candidate of dental plaque inhibition were not providing humoral reaction on rat body by observing the increase of IgG level.

Keyword: Glucanase enzymes, immunology, IgG, Indirect ELISA,

Abstrak

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Daftar Pustaka

  1. Ajjan N., Vaccination.. Lyon-France:, Institute Marieux, 1986.
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