Detail Article

Conservative Dentistry Journal / Vol. 4. No. 2. 2014-01: 27 - 33


The determination of minimal inhibitory concentration (MIC) and minimal Bactericidal concentration (MBC) against biofilm of Porphyromonas gingivalis


Penentuan konsentrasi hambat minimal (KHM) dan konsentrasi bunuh minimal (KBM) ekstrak Propolis terhadap biofilm bakteri Porphyromonas gingivalis

  1. Maria Istiqomah Marini
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  2. Dian Agustin W
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  3. Febriastuti Cahyani
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Abstract

Background: Porphyromonas gingivalis become one gram negative bacteria found in endodontic infections. Porphyromonas gingivalis is a persistent microorganisms that can cause failure of root canal treatment, because the bacteria can form biofilms that are more resistant microorganisms defense against antibiotics and the immune response. Back to nature, there is alternative material, one of them is propolis. Propolis active compounds like flavonoids, essential oils, terpenoids, saponins, and propolis ethanolic extracts are significant to exhibited antimicrobial activity. Propolis antimicrobial effects is directly proportional to its concentration. Purpose: The aim of this study is to know the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of propolis extract against biofilm of Porphyromonas gingivalis. Method:  This study was an in-vitro experimental research laboratory with the post test only control group design. Propolis extracted by ethanol 70% on maceration method and dilute into several concentrations. Value of MIC and MBC were done by counting the biofilm bacteria colony in Tryptic Soy Agar (TSA) with Drop Plate Miles Misra method. Growth of bacteria colonies is calculated manually in colony forming unit (CFU)/ml. Result: There was no effect from the smallest concentration to the biggest concentration of propolis extract against biofilm of Porphyromonas gingivalis. Conclusion: There wasn`t MIC and MBC of propolis extract to the biofilm of Porphyromonas gingivalis.

Keyword: Propolis extract, Biofilm, Porphyromonas gingivalis, MIC, MBC,

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