Detail Article

Oral Biology Dental Journal / Vol. 5. No. 1. 2013-01: -


The Success of FTATM Paper Extraction Method on Bitemarks DNA Identification Locus TH01, vWA in Odontology Forensic


Keberhasilan Metode Ekstraksi FTATM Paper pada pemeriksaan DNA Bitemark Lokus TH01,vWA di Bidang Kedokteran Gigi Forensik

  1. Anita Yuliati
    Department of Dental Material, Faculty of Dentistry Airlangga University, Surabaya - Indonesia
    aruslan@hotmail.com
  2. Michael Natanael Winarko
    mhs S 1
    -
  3. Agung Sosiawan
    Staf Pengajar Departemen Biologi Oral Fakultas Kedokteran Gigi Universitas Airlangga
    -
  4. Sophia Eleonora
    Staf Pengajar Departemen Biologi Oral Fakultas Kedokteran Gigi Universitas Airlangga
    -

Abstract

Backgroud. Forensic case often encountered bitemark evidence that was found on the victim’s or perpetrator’s body. Bitemark can be compared with the tooth model of suspected person physically. However, this technique may cause subjective interpretations. Therefore, an alternative technique to identify the bitemark case was used. The biological evidence from bitemark may be taken by irrigating the bitemark to collect the saliva and buccal epithelium deposited during biting so that the DNA from the suspected person can be identified. In the fact DNA material that has been found was very limited and might be degraded, less both in quantity and quality. Due to that, an effective method to extract the DNA material is needed. Purpose. Proving the success of DNA extraction with FTATMPaper method through PCR-Short Tandem Repeat (STR) on TH01,vWA loci. Method. Blood and bitemark samples were taken from 5 persons, each sample was exctracted with FTATMPaper method. When a sample is applied to the FTATMPaper the cell membranes are lysed, the nucleic acids are released and the DNA is entrapped within the fibres of the cards’ matrix. Purification/extraction of the DNA is simple (only requiring a series of wash steps). This means that DNA quantitation need not and cannot be measured/performed for samples extracted from FTATMPaper. DNA samples were amplified with PCR which vWA and TH01 loci were as the target. Visualizing band appearances were using 6% polyacrylamide gel electrophoresis. Result. Electrophoresis visualisations show band appearances of vWA and TH01 loci from blood and bitemark samples which mean the FTATMPaper method can be used for bitemark. Conclusion. It was proven that FTATMPaper extraction can be used effectively to extract DNA from bitemark.

Keyword: bitemark, FTATMPaper, vWA, and TH01 loci,

Abstrak

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Daftar Pustaka

  1. Atmadja, D.S., Peranan sidik jari DNA pada bidang kedokteran forensik. Materi Workshop DNA finger printing. Yogyakarta., Universitas Gadjah Mada, 2005.

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